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Oxidation of dihydronicotinamide adenine dinucleotide by a flavin derivative of polyethylenimine
Author(s) -
Spetnagel Wayne J.,
Klotz Irving M.
Publication year - 1978
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1978.360170704
Subject(s) - chemistry , polyethylenimine , nicotinamide adenine dinucleotide , flavin adenine dinucleotide , nad+ kinase , flavin group , hydrogen peroxide , catalysis , riboflavin , redox , photochemistry , flavoprotein , cofactor , kinetics , combinatorial chemistry , organic chemistry , biochemistry , enzyme , transfection , gene , physics , quantum mechanics
A modified Polyethylenimine has been prepared that has riboflavin attached to it, as well as hydrophobic groups. The catalytic efficiency toward oxidation of6 dihydronicotinamide adenine dinucleotide (NADH) by this flavopolymer is more than 100‐fold greater than observed with small‐molecular riboflavin. The products of the reaction in aerobic solution have been established to be the oxidized nicotinamide NAD + and hydrogen peroxide. The kinetics fit a Michaelis‐Menten mechanism. Thus, the versatility of modified polyethylenimines as catalysts has been extended from hydrolyses and decarboxylations to oxidation‐reduction reactions also.