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Self‐association phenomenon specific to the copper complexes with ADP, dADP, and IDP
Author(s) -
Gabriel Marc,
Larcher Dominique,
Thirion Christian,
Torreilles Jean,
De Paulet André Crastes
Publication year - 1978
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1978.360170604
Subject(s) - chemistry , pyrophosphate , nucleotide , crystallography , molecule , oligomer , copper , circular dichroism , adenine nucleotide , organic chemistry , biochemistry , gene , enzyme
Circular dichroism spectra between 200 and 800 nm are presented for solutions of nucleotides with pyrophosphate group derived from adenine and inosine in the presence of the Cu 2+ cation. A study as a function of the pH, temperature, and concentration has shown the existence of a self‐association, which implies a strong interaction between the bases of the two nucleotides. The interpretation of the dichroic spectra allows us to show that two oligomer species are formed successively in low concentrations in the presence of the Cu 2+ cation. The first, constituted by Cu(ADP) − molecules, appears at pH 5.3 and gives rise to a nonconservative excitonic term. The second, responsible for a conservative excitonic term, is a heterooligomer formed at pH 7.3 by the assembling of a Cu(ADP) − and a Cu(ADP)(OH) 2− molecule. Furthermore, this self‐association is destabilized if the nucleotide derived from adenine is replaced by the corresponding deoxyribonucleotide or by IDP.