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Application of thin film dialysis to the determination of equilibrium constants
Author(s) -
Russo Salvatore F.,
Engel Randall G.
Publication year - 1978
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1978.360170403
Subject(s) - dialysis , chemistry , constant (computer programming) , thermodynamics , equilibrium constant , thin film , series (stratigraphy) , physics , quantum mechanics , surgery , computer science , medicine , paleontology , biology , programming language
A theoretical basis for thin‐film dialysis involving binding between a ligand and nondialyzing species is presented. A general differential equation that applies to the case of equivalent, noninteracting sites is derived relating [ A ] F , [ A ] T , [ P ] T , and K . Numerical solutions to this equation are used to develop a series of escape curves corresponding to specific values of the parameters [ P ] T , [ A ] i , K , and k 0 . A general method for determining an equilibrium binding constant from thin‐film dialysis data is given. A comparison of thin‐film dialysis results predicted by this theory with literature data shows essential agreement.