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Binding of azide ion to methemoglobin at elevated temperatures and the reality of the “compensation” temperature
Author(s) -
Anusiem A. C. I.,
Ogunmola G. B.,
Beetlestone J. G.
Publication year - 1977
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1977.360161203
Subject(s) - chemistry , methemoglobin , azide , atmospheric temperature range , ion , hemoglobin , ligand (biochemistry) , range (aeronautics) , inorganic chemistry , crystallography , thermodynamics , organic chemistry , biochemistry , receptor , physics , materials science , composite material
We have studied the binding of azide ion to ferrihemoglobin at elevated temperatures. Up to a temperature of 45°C there is no difference in the ligand binding behavior of hemoglobin when compared with the results obtained at lower temperatures. The compensation temperature Tc of 290.6 ± 5.3°K, obtained in this study within the temperature range 303–318°K, confirms that the compensation pattern obtained by Lumry and Rajender is not dependent on the temperature range of the experiment but an intrinsic property of the protein conformation.