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DNA melting temperatures and renaturation rates in concentrated alkylammonium salt solutions
Author(s) -
Orosz Jerry M.,
Wetmur James G.
Publication year - 1977
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1977.360160603
Subject(s) - chemistry , melting point , salt (chemistry) , solvent , dna , melting temperature , hydrocarbon , freezing point , organic chemistry , thermodynamics , biochemistry , materials science , physics , composite material
DNA melting temperatures and renaturation rates have been determined for Me 2 Et 2 NBr and a series of RMe 3 NBr and REt 3 NBr solvents where R is a linear hydrocarbon chain. The point of independence of DNA melting temperature on base composition has been investigated for each solvent system. Renaturation rates are compared with those found in other concentrated salt solutions. Solvent mixtures which accelerate DNA renaturation have also been investigated.