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The organization and repair of DNA in the mammalian chromosome. II. A gradient‐shape‐induced speed dependence affecting DNAs larger than T 4 DNA
Author(s) -
Lange Christopher S.,
Martin Patricia,
Ferguson Pamm,
Resnick Michael A.
Publication year - 1977
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1977.360160510
Subject(s) - sedimentation , chemistry , dna , sucrose gradient , centrifugation , concentration gradient , biophysics , rotor (electric) , chromosome , sediment , physics , biology , biochemistry , chromatography , gene , membrane , paleontology , quantum mechanics
Abstract The addition of a lytic layer to a preformed linear sucrose gradient induces a temporary (up to half a day) initial gradient of considerable steepness which retards the sedimentation of large (>T 4 ) DNAs. Centrifugation at sufficiently slow angular velocities permits the temporary initial gradient to disappear and therefore the sedimentation distance increases, yielding a rotor speed dependent effect on sedimentation distance. Gradients which are free of this effect are described and shown to permit mouse leukemia cell DNA to sediment independently of rotor speed (5–30 krpm).