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The organization and repair of DNA in the mammalian chromosome. I. Calibration procedures and errors in the determination of the molecular weight of a native DNA
Author(s) -
Lange Christopher S.,
Liberman Daniel F.,
Clark Roger W.,
Ferguson Pamm
Publication year - 1977
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1977.360160509
Subject(s) - chemistry , dna , exponent , variance (accounting) , value (mathematics) , reproducibility , calibration , statistics , chromatography , biochemistry , mathematics , philosophy , linguistics , accounting , business
The semiautomated sucrose gradient system of Lange and Liberman (1974) ( Anal. Biochem. 59 , 129–145) has been used to determine an accurate value of the exponent k of the Burgi and Hershey equation (1963) (Biophys. J. 3 , 309–321) under conditions of 1 M salt. A complete analysis of the variance of k was done for data from both systems. The results lead to the conclusion that the value k = 0.401 (±0.012 for reproducibility; ±0.019 for total error) obtained here is considerably more accurate than that of Burgi and Hershey (even when corrected for DNA concentration effects and the improved size estimates of T 4 DNA size). The use of the Burgi and Hershey relationship and a T 4 DNA metric to determine molecular weights of mammalian DNA requires the utmost precision in the exponent if useful estimates are to be obtained. This also has implications for double‐strand breakage and repair estimates.