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Intensity fluctuation spectroscopy of deoxyribonucleic acid (DNA). I. Temperature profile of bacteriophage N1‐DNA
Author(s) -
Schmidt R. L.,
Boyle J. A.,
Mayo John A.
Publication year - 1977
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1977.360160208
Subject(s) - chemistry , hyperchromicity , dna , diffusion , spectroscopy , bacteriophage , intensity (physics) , laser , crystallography , optics , thermodynamics , biochemistry , physics , escherichia coli , quantum mechanics , gene
Intensity‐fluctuation laser‐light‐scattering spectroscopy has been used to characterize the temperature dependence of the translational diffusion coefficient through the helix–coil region for the DNA isolated from bacteriophage N1. The diffusion coefficient does not change smoothly, but exhibits fine structure detail similar to that seen in the uv absorption hyperchromicity. These changes are related to the heterogeneous distribution of bases along the N1‐DNA chain.