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Hyperfine structure in melting profile of bacteriophage lambda DNA
Author(s) -
Gotoh Osamu,
Husimi Yuzuru,
Yabuki Sadato,
Wada Akiyoshi
Publication year - 1976
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1976.360150406
Subject(s) - polynucleotide , dna , bacteriophage , lambda , chemistry , hyperfine structure , melting temperature , nucleic acid thermodynamics , homogeneous , nucleic acid , crystallography , base sequence , thermodynamics , physics , materials science , biochemistry , atomic physics , optics , escherichia coli , composite material , gene
A high‐resolution plotter of differential melting profiles of DNA, RNA, or related biopolymers with an on‐line mini‐computer is described. With this device, more than 15 transition steps were identified in the thermal melting profile of DNA from bacteriophage lambda. These fine structures were found to be reproducible, and some of them disappear in the deletion mutant. To Examine the melting profile, computer simulations for several hypothetical polynucleotide sequences were performed, and compared with experimental data. The sharp peaks that appeared in the differential melting profile of λ DNA may come from some homogeneous sequences of 500 bases or longer.