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Molecular forces in ribosomes and polynucleotide complexes
Author(s) -
Bresler S. E.,
Saminsky E. M.,
Chernajenko V. M.
Publication year - 1976
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1976.360150405
Subject(s) - chemistry , polynucleotide , ternary complex , ribosome , magnesium , stability constants of complexes , hydrogen bond , ternary operation , molecule , chelation , inorganic chemistry , ion , stereochemistry , crystallography , organic chemistry , rna , biochemistry , enzyme , computer science , programming language , gene
The stability constant of the complex of tRNA with 50 S subunits of ribosomes was compared in ordinary and heavy water. A considerable effect (about fourfold) was observed, showing the importance of hydrogen bonds in this interaction. In addition, the isotope effect of complementary polynucleotide interaction was measured for two examples. In the case of the binary complex of heptainosinic acid oligomers with poly(C) in the presence of 10 −3 M MgCl 2 , the transfer from ordinary to heavy water gave an increase of the stability constant of about 5%. But in the case of a ternary complex of hexaadenylic acid with poly(U) under the same conditions, the stability constant in D 2 O increased threefold. The isotope effect depends strongly on magnesium ion concentration and is possibly due to some specific mechanism of magnesium ion complexing involving water molecules.