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Cooperative ligand binding by ferrihemoglobin: An experimental artifact?
Author(s) -
Anusiem Alphonso C. I.,
Beetlestone John G.
Publication year - 1976
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1976.360150216
Subject(s) - chemistry , methemoglobin , ligand (biochemistry) , artifact (error) , perturbation (astronomy) , crystallography , computational chemistry , biochemistry , receptor , hemoglobin , neuroscience , physics , quantum mechanics , biology
We have carried out a detailed study of ligand binding of ferrihemoglobins under various conditions. Our results show that n varies with time and that this variation is paralleled by changes in the spectrum of methemoglobin. This suggests some form of structural perturbation. The time‐dependent value of n is discussed in terms of the observed spectral changes accompanying prolonged equilibration.