Cooperative ligand binding by ferrihemoglobin: An experimental artifact? | Zendy

Anusiem Alphonso C. I. | Zendy; Beetlestone John G. | Zendy

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Cooperative ligand binding by ferrihemoglobin: An experimental artifact?

Author(s) -

Anusiem Alphonso C. I.,

Beetlestone John G.

Publication year - 1976

Publication title -

biopolymers

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.556

H-Index - 125

eISSN - 1097-0282

pISSN - 0006-3525

DOI - 10.1002/bip.1976.360150216

Subject(s) - chemistry , methemoglobin , ligand (biochemistry) , artifact (error) , perturbation (astronomy) , crystallography , computational chemistry , biochemistry , receptor , hemoglobin , neuroscience , physics , quantum mechanics , biology

We have carried out a detailed study of ligand binding of ferrihemoglobins under various conditions. Our results show that n varies with time and that this variation is paralleled by changes in the spectrum of methemoglobin. This suggests some form of structural perturbation. The time‐dependent value of n is discussed in terms of the observed spectral changes accompanying prolonged equilibration.

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