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High‐resolution nmr study of yeast tRNA CUA Leu and the native and denatured conformers of yeast tRNA UUG Leu
Author(s) -
Rordorf B. F.,
Kearns D. R.,
Hawkins Erin,
Chang S. H.
Publication year - 1976
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1976.360150210
Subject(s) - transfer rna , conformational isomerism , chemistry , protein tertiary structure , crystallography , yeast , nuclear magnetic resonance spectroscopy , molecule , base pair , resolution (logic) , stereochemistry , rna , dna , biochemistry , organic chemistry , artificial intelligence , computer science , gene
The high‐resolution nmr spectrum of baker's yeast tRNA CUA Leu , a recently sequenced non‐denaturable tRNA, has been compared with the spectra of the native and denatured conformers of the closely related species tRNA UUG Leu . Because of the presence of many common base pairs in the different tRNA's, it is possible to assign most of the low‐field resonances to specific secondary‐structure base pairs. A comparison of the observed positions of the various resonances with those predicted by a semiempirical ring‐current shift theory shows a root‐mean‐square deviation of 0.14, 0.11, and 0.12 ppm for tRNA UUG Leu(native), and tRNA UUG Leu(denatured), respectively. These results support the ring‐current shift theory currently used to interpret the low‐field nmr spectra of the tRNA molecules. Differences between the predicted and observed positions of some resonances provide new evidence for higher order effects such as shifts from second nearest neighbors, anomalous shifts exerted by G·U base pairs, and tertiary‐structure effects. A model that was previously proposed for the denatured conformer of tRNA UUG Leuis also supported.