Premium
Thickness and density of protein films by optical mixing spectroscopy
Author(s) -
Uzgiris E. E.,
Fromageot H. P. M.
Publication year - 1976
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1976.360150204
Subject(s) - chemistry , adsorption , mixing (physics) , spectroscopy , glycoprotein , bovine serum albumin , polystyrene , molecule , albumin , protein adsorption , membrane , spheres , analytical chemistry (journal) , biophysics , chromatography , polymer , biochemistry , organic chemistry , physics , quantum mechanics , astronomy , biology
The adsorption of protein films on polystyrene latex spheres was studied by optical mixing spectroscopy. With this technique, we show that both the hydrodynamic thickness of protein films and their optical density can be measured. Thus, we found that films of the glycoproteins isolated from the human erythrocyte membrane were four times as thick as films of either human serum albumin or bovine serum albumin for about the same surface coverage. This result suggests an end‐on orientation for the adsorbed glycoprotein molecules, which is consistent with the model proposed by others for the orientation of these molecules at the surface of the red blood cell itself.