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Kinetic study of cooperative binding reaction of toluidine blue to poly(α, L ‐glutamic acid) by means of the electric‐field pulse method
Author(s) -
Ushio Hidetoshi,
Yasunaga Tatsuy,
Sano Takayuki,
Tsuji Yoshikuni
Publication year - 1976
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1976.360150113
Subject(s) - chemistry , glutamic acid , toluidine , titration , ionic bonding , polymer , amino acid , pulse (music) , ionic strength , stereochemistry , polymer chemistry , organic chemistry , biochemistry , ion , aqueous solution , electrical engineering , engineering , detector
Interaction of toluidine blue with helical and randomly coiled poly(α, L ‐glutamic acid) was studied with absorption spectra, titration, and electric‐field pulse measurements. The obtained values of various parameters for the helical form of poly(α, L ‐glutamic acid)differed from those for its coiled form. The difference of parameters in these two forms of poly(α, L ‐glutamic acid) was attributed to differences of the binding mechanism in both forms. Furthermore, the binding of toluidine blue to poly(α, L ‐glutamic acid) was considered to be due to hydrogen binding in the helical conformation and ionic interaction in the coiled conformation of the polymer.