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Electron microscopy of ribosomal RNA mounted by adsorption in the absence of protein
Author(s) -
Johnson Thomas R.,
Caston J. Douglas
Publication year - 1975
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1975.360140715
Subject(s) - ribosomal rna , rna , adsorption , chemistry , monolayer , electron microscope , molecule , ribosomal protein , crystallography , 5s ribosomal rna , biophysics , 18s ribosomal rna , biochemistry , ribosome , biology , physics , organic chemistry , optics , gene
A method is described for visualizing ribosomal RNA in the electron microscope by an adsorption technique. The visual configuration of the molecules is sensitive to the composition of the medium from which they are adsorbed, and is reproducible under a given set of conditions. Regular structural features occur in the larger rRNA species similar to those observed with protein monolayer techniques. The dimensions of adsorbed molecules, however, are more consistent with current models of ribosomal RNA as it exists in solution.