The magnitude of intramolecular DNA optical absorbance with variation in [Na + ] and GC base composition: For special application to DNA reassociation studies | Zendy

Marx Kenneth A. | Zendy

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The magnitude of intramolecular DNA optical absorbance with variation in [Na + ] and GC base composition: For special application to DNA reassociation studies

Author(s) -

Marx Kenneth A.

Publication year - 1975

Publication title -

biopolymers

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.556

H-Index - 125

eISSN - 1097-0282

pISSN - 0006-3525

DOI - 10.1002/bip.1975.360140517

Subject(s) - absorbance , chemistry , intramolecular force , dna , analytical chemistry (journal) , base (topology) , magnitude (astronomy) , composition (language) , base pair , chromatography , stereochemistry , biochemistry , mathematical analysis , physics , linguistics , mathematics , philosophy , astronomy

The magnitude of intramolecular DNA optical absorbance is presented as a function of [Na + ] and GC composition of the DNA. The data are presented as a ratio of absorbances A 0 T r/ A 0 T dfor the DNA at the denaturing temperature ( T d ) and renaturing temperature ( T r ) under the given conditions. All ratios were determined for T r corresponding to the temperature optimum ( T m − T r = 25°C) in DNA reassociation rate. This fact, coupled with the convenient A 0 T r/ A 0 T dratio representation, permits the quick estimation of the magnitude of this optical effect in DNA reassociation reactions over a wide range of experimental conditions.

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