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Fluorescence correlation spectroscopy and nonideal solutions
Author(s) -
Phillies George D. J.
Publication year - 1975
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1975.360140306
Subject(s) - fluorescence correlation spectroscopy , chemistry , fluorescence , macromolecule , diffusion , spectroscopy , fluorescence spectroscopy , fluorescence cross correlation spectroscopy , membrane , analytical chemistry (journal) , correlation coefficient , thermodynamics , molecule , chromatography , optics , organic chemistry , biochemistry , physics , statistics , mathematics , quantum mechanics
The information that may be obtained from a fluorescence correlation spectroscopic study of a nonideal solution is considered. If all of the macromolecules in a two‐component solution are fluorescently labeled, the mutual diffusion coefficient will be measured. If only a few of the macromolecules in a solution are fluorescently labeled, the tracer diffusion coefficient will be obtained. Two nonideal systems that probably may usefully be studied with fluorescence correlation spectroscopy are proposed. The application of fluorescence correlation spectroscopy to studies of lateral diffusion in biological membranes is discussed; the form of the contribution to the fluorescence correlation spectrum of bulk motion within a membrane is noted.