Premium
Fluorescence correlation spectroscopy and Brownian rotational diffusion
Author(s) -
Aragón S. R.,
Pecora R.
Publication year - 1975
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1975.360140110
Subject(s) - rotational diffusion , fluorescence correlation spectroscopy , chemistry , fluorescence , autocorrelation , brownian motion , diffusion , relaxation (psychology) , correlation function (quantum field theory) , range (aeronautics) , rotational correlation time , spectroscopy , fluorescence spectroscopy , nuclear magnetic resonance , analytical chemistry (journal) , molecular physics , physics , optics , thermodynamics , materials science , molecule , quantum mechanics , psychology , social psychology , statistics , mathematics , organic chemistry , chromatography , dielectric , composite material
A theroy relating rotational Brownian motion to the time autocorrelation function of the intensity of radiation from a fluorescent system composed of spherical rotors is presented. The calculation shows three relaxation times, two associated with the rotational diffusion, and the third associated with the natural decay of the fluorescence. The correlation function contains terms that relax independently of the fluorescence decay time, thus arbitrarily extending the time range over which rotational diffusion can be studied by fluorescence.