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The ESR determination of protein α‐helicity
Author(s) -
Lvov K. M.,
Kim Yu. A.
Publication year - 1975
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1975.360140107
Subject(s) - helicity , chemistry , globular protein , kinetics , paramagnetism , polypeptide chain , crystallography , biochemistry , physics , amino acid , condensed matter physics , particle physics , quantum mechanics
Experimental data are summarized on decay kinetics of uv‐induced paramagnetic centers in globular proteins. The PC decay rate in the absence of oxygen in α helical regions is shown to differ always by about two orders from that in nonhelical regions of the polypeptide chain. This effect can be utilized to estimate the degree of α helicity in the dry state, in solution, and in the frozen solution of proteins.
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