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Molecular weight and shape of the phycocyanin hexamer
Author(s) -
Kato Masanori,
Lee Wylie I.,
Eichinger B. E.,
Schurr J. M.
Publication year - 1974
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1974.360131111
Subject(s) - random hexamer , chemistry , sedimentation coefficient , monomer , radius , ammonium sulfate , crystallography , intrinsic viscosity , chromatography , organic chemistry , polymer , computer security , enzyme , computer science
The hexamer of phycocyanin from Phormidium luridum has been isolated and purified by ammonium sulfate fractionation and gel chromatography. The protein is characterized by the sedimentation constant S ° 20, w = 10.2S, the diffusion coefficient D 20, w = 4.73 × 10 −7 cm 2 /sec, and intrinsic viscosity [η] = 3.89 ml/g. The molecular weight of the aggregate is 209,000. The shape and dimensions of the hexamer are discussed in terms of a model consisting of subunits arranged with C 6 symmetry. The monomers, assumed to be spherical, are found to have a radius of 22 Å, and the diameter across the hexamer is 132 Å. The latter figure agrees closely with dimensions observed in electron micrographs.