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Measurement of diffusion coefficients of meningococcal polysaccharide by optical mixing spectroscopy. I. A preliminary characterization on the aggregation of the Group C polysaccharide
Author(s) -
Chen F. C.,
Tscharnuter W.,
Schmidt D.,
Chu B.,
Liu T. Y.
Publication year - 1974
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1974.360131110
Subject(s) - chemistry , dispersity , diffusion , monomer , analytical chemistry (journal) , polysaccharide , rotational diffusion , dimer , correlation function (quantum field theory) , chromatography , polymer , thermodynamics , polymer chemistry , physics , organic chemistry , molecule , optoelectronics , dielectric
The angular distribution of scattered intensity and decay times of concentration fluctuations have been measured by means of digital photon counting and single‐clipped photon correlation for solutions of Group C meningococcal polysaccharides at 31°C. The z ‐average diffusion coefficient 〈 D 〉 z and its second moment 〈 D 2 〉 z have been determined from the time‐dependent correlation function using the cumulant expansion technique. Very low observed values of 〈 D 〉 z and the tremendous width of the polydispersity index, which is the z ‐average normalized variance, suggest a higher degree of aggregation than the monomer–dimer type self‐association at finite concentrations.