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Buoyant densities of natural and synthetic DNA's in CsCl and Cs 2 SO 4 considered as sequence‐dependent properties
Author(s) -
Gray Donald M.,
Gray Carla W.,
Ratliff Robert L.,
Smith David A.
Publication year - 1974
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1974.360131108
Subject(s) - dna , buoyant density , chemistry , sequence (biology) , base pair , dna sequencing , thermodynamics , crystallography , physics , biochemistry
Abstract The buoyant densities of natural and synthetic DNA's can be accurately interrelated if second‐neighbor influences are taken into account. We derive the following expressions, based partly on the buoyant densities of six synthetic DNA's, for the buoyant densities ρ (g/cm 3 ) of DNA's having random sequences. In CsCl,\documentclass{article}\pagestyle{empty}\begin{document}$$ \rho = 1.659 + 0.099H_{\rm G} - 0.065\,H_{\rm G} ^2 + 0.080\,H_{\rm G} ^3 $$\end{document} and in Cs 2 SO 4 ,\documentclass{article}\pagestyle{empty}\begin{document}$$ \rho = 1.417 + 0.011\,H_{\rm G} - 0.004\,H_{\rm G} ^2 + 0.035\,H_{\rm G} ^3 $$\end{document} .In these equations, H G is the mole fraction of G : C base pairs in the DNA and the buoyant densities are calculated relative to densities for E. coli DNA of 1.703 and 1.426 (g/cm 3 ) in CsCl and Cs 2 SO 4 , respectively.