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Effects of microscopic and macroscopic viscosity on the rate of renaturation of DNA
Author(s) -
Chang ChiangTung,
Hain Timothy C.,
Hutton James R.,
Wetmur James G.
Publication year - 1974
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1974.360130915
Subject(s) - chemistry , viscosity , solvent , polymer , intrinsic viscosity , salt (chemistry) , ammonium perchlorate , volume (thermodynamics) , perchlorate , ammonium , relative viscosity , thermodynamics , organic chemistry , ion , physics , thermal decomposition
The effect of solvent viscosity on DNA renaturation rates has been investigated as a function of temperature for a number of solvent systems. The results are all consistent with a microscopic viscosity limitation of the rate determining step. Rates of renaturation in perchlorate and quaternary ammonium salt solutions are also discussed. Increasing the macroscopic viscosity with dissolved neutral or anionic polymers increases, rather than decreases, renaturation rates due to the excluded volume of the dissolved polymers.