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The state of water on hydrated collagen as studied by pulsed NMR
Author(s) -
Fung B. M.,
Witschel John,
McAmis Lavon L.
Publication year - 1974
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1974.360130910
Subject(s) - chemistry , adsorption , isotropy , molecule , relaxation (psychology) , analytical chemistry (journal) , chromatography , organic chemistry , psychology , social psychology , physics , quantum mechanics
The spin‐lattice relaxation time ( T 1 ) of water adsorbed on collagen fibers was determined at six frequencies and temperatures varying from 25° to −80°C. Care was taken to eliminate the contributions to the signal of protons other than those in the adsorbed water. Quantitative calculations were made on T 1 and the results were compared with the experimental data. It is suggested that a maximum of about 0.50–0.55 g water per g collagen forms a hydration layer, which cannot be frozen down to −90°C and exhibits a distribution of motional correlation times. For collagen samples containing a larger quantity of adsorbed water, the additional water molecules behave like ordinary isotropic water, having a single correlation time and a freezing temperature of about −10°C.