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Energy transfer in tRNA Phe (Yeast). The solution structure of transfer RNA
Author(s) -
Blumberg W. E.,
Dale R. E.,
Eisinger J.,
Zuckerman D. M.
Publication year - 1974
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1974.360130808
Subject(s) - chemistry , acceptor , transfer rna , chromophore , chemical physics , photochemistry , rna , physics , quantum mechanics , biochemistry , gene
In the preceding paper, the dependence of the dipolar orientation factor κ 2 , which governs long‐range (Förster) energy transfer, on the relative orientation and reorientational freedom of the donor and acceptor chromophores is examined. The analysis given there is employed to derive minimum and maximum values of 〈κ 2 〉 for energy transfer from the Y base of tRNA Phe (yeast) to an acriflavine acceptor attached to the 3′ terminus of the tRNA using estimates for the reorientational freedom of the donor derived from a combination of steady‐state and time‐dependent polarized emission measurements. The acceptor motion is estimated from data in the literature. From these limiting values of 〈κ 2 〉 and Beardsley and Cantor's determination of the long range energy‐transfer efficiency for this donor–acceptor pair, the interchromophore distance R is calculated to be between 34 and 61 Å, this range being independent of the particular model chosen to described the reorientational freedom of the donor and acceptor. While this large range in R is of little help in establishing the solution structure of tRNA, the analysis illustrates the potential of polarized energy‐transfer experiments, particularly when transfer depolarization experiments are possible.