Premium
Intramolecular distances determined by energy transfer. Dependence on orientational freedom of donor and acceptor
Author(s) -
Dale Robert E.,
Eisinger Josef
Publication year - 1974
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1974.360130807
Subject(s) - intramolecular force , chemistry , acceptor , degrees of freedom (physics and chemistry) , orientation (vector space) , macromolecule , energy transfer , chemical physics , molecule , substrate (aquarium) , transfer (computing) , resonance (particle physics) , förster resonance energy transfer , range (aeronautics) , molecular physics , atomic physics , thermodynamics , stereochemistry , condensed matter physics , optics , physics , organic chemistry , geometry , materials science , mathematics , oceanography , computer science , composite material , biochemistry , parallel computing , fluorescence , geology
The dependence of Förster long‐range resonance energy transfer efficiency on the orientational freedom of donor D and acceptor A molecules attached to a macromolecular substrate is examined. The usefulness of polarized emission measurements in determining the mutual orientation as well as the degree of orientational freedom of D and A and thereby deriving maximum and minimum values for the D – A separation from the transfer efficieny is demonstrated.