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Molecular conformation of polysaccharides in solution. Changes in the optical rotation and in the elution pattern of gel filtration
Author(s) -
Hirano Shigehiro,
KondoIkeda Sumiyo
Publication year - 1974
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1974.360130707
Subject(s) - chemistry , aqueous solution , size exclusion chromatography , polysaccharide , elution , urea , chromatography , amylose , optical rotation , nitrocellulose , molecule , filtration (mathematics) , membrane , organic chemistry , biochemistry , starch , enzyme , statistics , mathematics
Molecular conformation of some polysaccharides in aqueous solution in evidenced by changes in the optical rotation and in the elution pattern of gel filtration. The changes in the specific rotation against that in water are expressed as a molar conformational value [ K ] D 21 : −495° for colominic acid in 1.0 N NaOH solution, and −180° for hyaluronate (HA), +85° for corneal keratin sulfate, and +234° for amylose in 8 M urea solution. The gel filtration of amylose and HA dissolved in 8 M urea solution shows elution patterns distinctly different from those dissolved in water. The phenomena are attributable to a molecular conformational transition of polysaccharide molecules in aqueous solution.