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Synthesis of a novel fluorescent polyriboadenylic acid analog by polynucleotide phosphorylase
Author(s) -
Tsou K. C.,
Yip K. F.
Publication year - 1974
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1974.360130513
Subject(s) - polynucleotide phosphorylase , chemistry , polynucleotide , fluorescence , escherichia coli , glycogen phosphorylase , polymerization , enzyme , biochemistry , stereochemistry , polymer , purine nucleoside phosphorylase , organic chemistry , physics , quantum mechanics , purine , gene
Poly(1, N 6 ‐etheno‐2‐aza‐adenylic acid) [poly(2‐aza‐εA)] was synthesized from 1, N 6 ‐etheno‐2‐aza‐adenosine 5′‐diphosphate (2‐aza‐εADP) and Escherichia coli polynucleotide phosphorylase. The values K m = 1.02 m M , V = 1.06 μmol hr −1 enzyme unit −1 were found for the polymerization reaction. In contrast to polyadenylic acid, this novel fluorescent polymer has a random structure in solution. The application of the 2‐aza‐εADP for localization of polynucleotide phosphorylase was also described.