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Dispersion effect on turbidimetric size measurement
Author(s) -
Cancellieri A.,
Frontali C.,
Gratton E.
Publication year - 1974
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1974.360130408
Subject(s) - dispersion (optics) , suspension (topology) , chemistry , turbidimetry , analytical chemistry (journal) , particle size , chromatography , optics , physics , mathematics , homotopy , pure mathematics
If dispersion effects are not taken into account, the dimensions of particles in suspension estimated from spectrophotometric data (slope of the log OD versus log λ plot) are too low. By measuring dispersion curves for the medium and for the suspension, a consistent estimate of the size of latex particles of different diameter is obtained from the OD data.