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Proton magnetic resonance study of complex formation between N 6 ‐methyladenosine and polyuridylic acid
Author(s) -
Bangerter Benedict W.,
Modestas Zivile
Publication year - 1974
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1974.360130309
Subject(s) - chemistry , proton magnetic resonance , n6 methyladenosine , stacking , uracil , monomer , hydrogen bond , crystallography , stereochemistry , proton , polymer , nuclear magnetic resonance , molecule , organic chemistry , biochemistry , dna , methyltransferase , physics , quantum mechanics , methylation
The interaction between N 6 ‐methyladenosine and polyuridylic acid in D 2 O solution at neutral pD has been studied as a function of temperature and N 6 ‐methyladenosine concentration by proton magnetic resonance spectroscopy. A rigid double‐stranded 1:1 complex is formed below ∼10°C, involving hydrogen‐bonded N 6 ‐methyladenine:uracil base‐pairing and stacking of the adenine bases. This complex is less stable than the 1:2 complex formed between adenosine and polyU, and involves a more rapid exchange of the monomer between free and polymer‐bound environments.