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Elution properties of inhomogeneous chromatographic systems
Author(s) -
Weiss George H.,
Dishon Menachem
Publication year - 1973
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1973.360121115
Subject(s) - chemistry , elution , chromatography , perturbation theory (quantum mechanics) , column (typography) , physics , mathematics , geometry , quantum mechanics , connection (principal bundle)
The first two moments of residence time in an inhomogeneous column are calculated by means of first passage time theory. These can be used to find the resolving power of the column for nearly equal amounts of protein. A recently developed perturbation theory allows the calculation of approximate elution profiles.