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Interaction of metal ions with polynucleotides and related compounds. XXII. Effect of divalent metal ions on the conformational changes of polyribonucleotides
Author(s) -
Shin Yong Ae
Publication year - 1973
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1973.360121103
Subject(s) - polynucleotide , chemistry , divalent , metal ions in aqueous solution , metal , crystallography , ion , nucleotide , stereochemistry , organic chemistry , biochemistry , gene
Changes in the conformation of poly(G), poly(C), poly(U), and poly(I) in the presence of divalent metal ions Mg 2+ , Ca 2+ , Mn 2+ , Co 2+ , Ni 2+ , Cu 2+ , Cd 2+ , and Zn 2+ have been measured by means of ORD and u.v. spectra. Mg 2+ and Ca 2+ ions stabilize helical structures of all the polynucleotides very effectively at concentrations several orders of magnitude lower than the effective concentration of Na + ion. Cu 2+ and Cd 2+ destabilize the helical structure of polynucleotides to form random coils. Zn 2+ , Ni 2+ , Co 2+ , and Mn 2+ ions do not behave in such a clear‐cut manner: they selectively stabilize some ordered structures, while destabilizing others, depending on the ligand strength of the nucleotide base as well as the preferred conformation of that polynucleotide.