Premium
Translational and rotational diffusion of tobacco mosaic virus from polarized and depolarized light scattering
Author(s) -
King T. A.,
Knox A.,
McAdam J. D. G.
Publication year - 1973
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1973.360120817
Subject(s) - chemistry , tobacco mosaic virus , rotational diffusion , scattering , diffusion , rayleigh scattering , spectral line , analytical chemistry (journal) , laser linewidth , molecule , molecular physics , atomic physics , nuclear magnetic resonance , optics , physics , virus , chromatography , thermodynamics , laser , organic chemistry , virology , astronomy , biology
The translational and rotational dynamics of tobacco mosaic virus in sodium phosphate buffer (pH =7.5) solutions has been investigated by polarized and depolarized light scattering Rayleigh linewidth studies. For concentrations ranging from 1.75 × 10 −4 g ml −1 to 0.25 × 10 −4 g ml −1 the translational diffusion coefficient (D T ) has been found to be slightly concentration dependent and extrapolation to zero concentration gives D 0 20°C = 0.34 ± 0.01 × 10 −7 cm 2 S −1 . A full analysis of the polarized spectra obtained at high and low scattering angles and the depolarized spectra at near zero scattering angles has enabled these techniques to be compared and the rotational diffusion constant D R to be determined. At a solution concentration of 1.75 × 10 −4 g ml −1 a mean value is found to be D R 20°C = 350 ± 30s −1 . These values of D T and D R are in approximate agreement with calculations based on models of the tobacco mosaic virus molecule as a cylindrical rod.