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Electrophoretic light scattering as a probe of reaction kinetics
Author(s) -
Berne B. J.,
Giniger Rina
Publication year - 1973
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1973.360120518
Subject(s) - chemistry , electric field , macromolecule , light scattering , globular protein , reaction rate , electrophoresis , kinetics , scattering , chemical kinetics , chemical reaction , field (mathematics) , rate equation , chemical physics , computational chemistry , optics , classical mechanics , chromatography , organic chemistry , physics , crystallography , quantum mechanics , biochemistry , mathematics , pure mathematics , catalysis
A new method is proposed for determining chemical rate contants in dimerization reactions of globular proteins. Light scattering from a solution of charged macromolecules in and applied electric field gives a series of bands whose widths can be used to deduce the reaction rate contants. This method should be applicable to other types of peactions. First order reactions are also considered.