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Histone IV binding of 3,4‐benzpyrene, pyrene, anthracene, and 9‐methylanthracene
Author(s) -
Small Enoch W.,
Craig A. Morrie,
Isenberg I.
Publication year - 1973
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1973.360120517
Subject(s) - chemistry , anthracene , pyrene , fluorescence , conformational change , histone , photochemistry , divalent , biophysics , stereochemistry , biochemistry , organic chemistry , dna , physics , quantum mechanics , biology
Ultraviolet absorption, fluorescence excitation and emission, and fluorescence anisotropy demonstrate that the polycyclic aromatic hydrocarbone 3,4‐benzpyrene, Pyrene, anthracene, and 9‐methylanthracene bind to histone IV after the sow conformational change in the presence of divalent phosphate anion. These hydrocarbons do not bind, within our limits of detection, to histone IV in the diosorderde form at low pH or to the form produced by the fast conformational change when the slow change is prevented. High anisotropy values for the bound hydrocarbon indicate a high degree of aggregation for the histone IV.