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Conformational studies of anthrax polypeptide, subtilis polypeptide, and synthetic poly‐γ‐ L glutamic acid
Author(s) -
Balasubramanian D.,
Kalita C. C.,
Kovacs J.
Publication year - 1973
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1973.360120513
Subject(s) - chemistry , circular dichroism , optical rotatory dispersion , polyglutamic acid , polymer , molecule , peptide , titration , chirality (physics) , glutamic acid , stereochemistry , polymer chemistry , amino acid , combinatorial chemistry , organic chemistry , biochemistry , nambu–jona lasinio model , chiral symmetry breaking , physics , quantum mechanics , quark
Poly‐γ‐ L ‐glutamic acid has been synthesized by the activated pentachlorophenyl ester polymerization method and the molecule weight of the polymer was found to be 16,000. Comparative conformational studies on the synthetic and on the native polyglutamic acid mbtained from B. anthracis and B. subtilis were carried out using optical rotatory dispersion, circular dichroism, peptide absorption spectrum, and titration data. These results show that poly‐γ‐glutamic acid does not exhibit any conformational order under the conditions of investigation. At low degrees of ionization, restriction of conformational freedom via random “hypercoiling” of the chain appears likely.