Premium
Hydrolysis of poly‐ L ‐lysine by pronase
Author(s) -
Sass R.,
Thiemann W.
Publication year - 1973
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1973.360120307
Subject(s) - pronase , lysine , chemistry , hydrolysis , amino acid , yield (engineering) , peptide , monomer , biochemistry , enzyme , trypsin , organic chemistry , polymer , materials science , metallurgy
Pronase E is able to hydrolyze poly‐ L ‐lysine in 100% yield into the monomer L ‐lysine. The experiments show an intermediate production of tri‐ L ‐lysine and di‐ L ‐lysine that are clipped off from the polymeric peptide. During the hydrolytic process a number of characteristic amino acids are released from the enzyme that may indicate its activation. Amino acids as well as mono‐ L ‐lysine are decomposed by pronase E after a period of several days.