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Single‐ and double‐strand breaks in 5‐bromouracil‐substituted DNA of B. subtilis and phage PBSH after irradiation with long‐wave length UV and their correlation to intramolecular energy transfer
Author(s) -
Mönkehaus Friedrich,
Köhnlein Wolfgang
Publication year - 1973
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1973.360120210
Subject(s) - chemistry , dna , intramolecular force , linear energy transfer , single strand , irradiation , double strand , stereochemistry , physics , dna damage , biochemistry , organic chemistry , ion , nuclear physics
5‐Bromouracil‐substituted DNA was isolated form B. Subtilis and phage PBSH. The three DNA fractions of Different densities (TT, TB, and BB) were irradiated with u.v. (313nm). The number of single‐strand and double‐strand breaks was determined. The breakage rates are given. It was found that in hybrid DNA (TB) double‐strand breaks occur depending linearly on dose. In BB–DNA the observed double‐Strand bresks can be divided into two fractions with a linear and quadratic dose dependence respectively. The results can be explained by assuming intramolecular energy transfer from the BU‐containing strand to its complementary strand.