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The effect of acridine orange on the structure of ribosomes
Author(s) -
Tal Moshe,
Rotem Haim,
Alfasi Matya,
Berg Robert A.
Publication year - 1973
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1973.360120116
Subject(s) - acridine orange , chemistry , ribosome , ionic strength , escherichia coli , acridine , ribosomal rna , ionic bonding , tris , biophysics , biochemistry , organic chemistry , ion , apoptosis , rna , biology , aqueous solution , gene
Exposure of Escherichia coli MRE‐600 ribosomes to acridine orange (AO) at low ionic strength (1m M Tris‐acetate pH 7.4) results in quantitative binding of the dye. Under our experimental conditions about a few hundred dye molecules can be bound to any one of the 30, 50, or 70‐S particles. AO causes the 30 and the 50‐S subunits to form ribosomal aggregates of approximate sedimentation constants of 70 and 100‐S.