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Magnitude of a finite equilibration effect in analytical gel chromatography
Author(s) -
Weiss George H.,
Ackers Gary K.
Publication year - 1972
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1972.360111012
Subject(s) - chemistry , magnitude (astronomy) , dispersion (optics) , chromatography , mechanics , physics , optics , astronomy
Previous analyses of solute behavior in analytical gel chromatography 1,2 have allowed for a finite equilibration time between stationary and mobile phases in estimating the magnitude of axial dispersion coefficients, but have ignored these effects in formulating the continuity equations for solute transport. We develop a more accurate theory including the equilibration effect, and show experimentally that it can be disregarded after approximately two minutes from the start of a small‐zone experiment.