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Viscosity of heparin as a function of dielectric constant and of desulfation
Author(s) -
Yuan L.,
Stivala S. S.
Publication year - 1972
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1972.360111008
Subject(s) - chemistry , dielectric , viscosity , solvent , polyelectrolyte , thermodynamics , salt (chemistry) , intrinsic viscosity , analytical chemistry (journal) , polymer , stereochemistry , organic chemistry , physics , quantum mechanics
The effect of dielectric constant ( D ) of the solvent on the viscosity of heparin was examined using the relation \documentclass{article}\pagestyle{empty}\begin{document}$ \eta _{{\rm sp}} /c = [\eta ]_\infty (1 + k/\sqrt c) $\end{document} , where [η] ∞ is the shielded intrinsic viscosity obtained by extrapolating \documentclass{article}\pagestyle{empty}\begin{document}$ \eta _{{\rm sp}} /c\,{\rm vs}{\rm . }\,1/\sqrt c ) $\end{document} to infinite concentration, and k is an interaction parameter independent of the dielectric constant of the solvent. This equation was previously reported by the authors 9 for describing the reduced viscosities of strong polyelectrolytes in salt‐free polar solvents. It was found that the [η] ∞ of heparin increases linearly with increasing dielectric constant of the solvent whereas the k values were, within experimental error, independent of D in the range 54.7 < D < 93.2 examined. Graded hydrolysis of heparin from its acid form (heparinic acid) at 57°C resulted in samples of varying degree of desulfation with corresponding decrease in biological activity. It was found that both [η] ∞ and k decrease with increasing desulfation.