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An analysis of the results for the binding of formaldehyde to polyuridylic acid
Author(s) -
Aylward N. N.
Publication year - 1972
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1972.360110705
Subject(s) - stacking , chemistry , formaldehyde , base (topology) , crystallography , computational chemistry , stereochemistry , organic chemistry , mathematical analysis , mathematics
Spectrophotometric measurements were made on the extent of binding of formaldehyde to polyuridylic acid under conditions of varying temperature and formaldehyde concentration. The data is interpreted in terms of a temperature‐dependent stacking of the bases in poly U at 20°, but not at 40°C. A theory of cooperative stacking is developed which considers the base residues to be either non‐bonded, non‐bonded and methylolated, or stacked. The results indicate essentially non‐cooperative base stacking under these conditions with an equilibrium constant for base stacking of 0.92 at 20°C.