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Circular dichroism of DNA: Temperature and salt dependence
Author(s) -
Studdert David S.,
Patroni Maria,
Davis Robert C.
Publication year - 1972
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1972.360110404
Subject(s) - circular dichroism , absorbance , chemistry , aqueous solution , vibrational circular dichroism , solvent , ultraviolet , analytical chemistry (journal) , crystallography , chromatography , optics , organic chemistry , physics
The temperature dependence of the circular dichroism and ultraviolet absorbance of calf thymus DNA and poly dAT were measured in aqueous solutions of various 1–1 electrolytes. Although there was very little change in absorbance, the circular dichroism changed dramatically as a function of both temperature and solvent. There is a correlation of the heat of hydration of the cation and the magnitude of the temperature dependence of the circular dichroism. Our results are interpreted in terms of a large number (possibly a continuum) of intermediate secondary structures.