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Thermal transitions in mixtures of polydeoxyribodinucleotides
Author(s) -
Hayes F. N.,
Lilly E. H.,
Ratliff R. L.,
Smith D. A.,
Williams D. L.
Publication year - 1970
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1970.360090911
Subject(s) - chemistry , enthalpy , absorbance , hyperchromicity , thermodynamics , oligomer , inverse , melting temperature , melting point , entropy (arrow of time) , analytical chemistry (journal) , chromatography , polymer chemistry , organic chemistry , dna , physics , biochemistry , geometry , mathematics , materials science , composite material
Oligo d(C‐A) and oligo d(T‐G) of known average lengths, prepared by a combination of chemical and enzymatic procedures, have been mixed in 0.02 M and 0.07 M Na + , and absorbance has been studied as a function of increasing temperature. The transitions have been analyzed for the temperature of maximum slope T m , the breadth of the transition, the value of the slope at T m , and the maximum hyperchromicity. Linear expressions have been found relating the inverse of the length in nucleotide units ( n −1 ) of the shorter oligomer, irrespective of its identity, to T m and also to the transition breadth. From a difference in slope between the T m versus n −1 expressions for the two molarities, the entropy and enthalpy of melting have been calculated as a function of n −1 .