Premium
Oligonucleotide interactions. III. Circular dichroism studies of the conformation of deoxyoligonucleolides
Author(s) -
Cantor Charles R.,
Warshaw Myron M.,
Shapiro Herman
Publication year - 1970
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1970.360090909
Subject(s) - chemistry , circular dichroism , dimer , oligonucleotide , crystallography , monomer , spectral line , stereochemistry , base pair , dna , cotton effect , biochemistry , polymer , organic chemistry , physics , astronomy
The circular dichroism (CD) spectra of the four usual deoxymononucleosides, all sixteen deoxydinucleotides, and a number of trinucleotides have been measured. The dimer spectra are quite different from the sum of the spectra of their constituent monomers. This indicates the presence of base‐stacked conformations analogous to those found for ribonucleoside diphosphates. The CD spectra of several deoxytrinucleotide diphosphates and single‐strand f 1 DNA can be calculated fairly well by using a semi‐empirical nearest‐neighbor approach. There is little or no effect of terminal phosphate or of salt concentration on the optical properties of most deoxy oligomers. The possibility of simultaneous analysis of mixtures of deoxypurine or deoxypyrimidine sequence isomers has been examined. This seems to be a viable approach for the analysis of purine runs but cannot promise much success for pyrimidine runs.