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Sedimentation of ribosomal ribonucleic acid from ribonuclease treated and untreated ribosomes
Author(s) -
Rodgers A.
Publication year - 1970
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1970.360090709
Subject(s) - formamide , ribosome , chemistry , rna , ribosomal rna , denaturation (fissile materials) , ribonuclease , aqueous solution , ultracentrifuge , ionic strength , nuclease , biophysics , crystallography , biochemistry , enzyme , organic chemistry , biology , nuclear chemistry , gene
The formation of a slowly sedimenting form of 23‐S ribosomal RNA from E. coli has been investigated by analytical ultracentrifugation and thermal denaturation in aqueous solution and in formamide. Evidence is presented that the slow form of 23‐S arises as a result of nucleate damage to the RNA in the 50‐S ribosome. The 30‐S ribosome (and 16‐S RNA), is unaffected. The slow form of 23‐S RNA cannot be demonstrated under conditions of complete denaturation in formamide, but only by partial denaturation in aqueous solution of low ionic strength (< 0.01 M Na). Apparent maintenance of the integrity of 23‐S RNA in formamide after nuclease treatment suggests that this may not be a simple linear molecule. An alternative model is suggested containing a circular element in the structure.