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Ionic polysaccharides. III. Dilute solution properties of hyaluronic acid fractions
Author(s) -
Cleland Robert L.,
Wang John L.
Publication year - 1970
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1970.360090706
Subject(s) - chemistry , virial coefficient , osmometer , intrinsic viscosity , diffusion , viscosity , thermodynamics , polymer , analytical chemistry (journal) , chromatography , organic chemistry , physics
Molecular weights by osmometry ( M̄ n ), light scattering ( M̄ w ), and sedimentation‐diffusion ( M sD ) were determined for selected fractions and unfractionated samples of hyaluronic acid in the molecular weight range 3 × 10 4 −1.7 × 10 6 . For the determination of M sD from the sedimentation coefficient s , a calibration procedure based on the data of Laurent, Ryan, and Pietruszkiewicz gave the following relations in 0.2 M NaCl: log ( s −1 ) 0 = 14.681 − 0.413 log M sD and log D 0 = −3.828 − 0.568 log M sD , where subscript, zero refers to zero concentration and D is the diffusion coefficient. The average Mandelkern‐Flory parameter β in 0.2 M NaCl is 3.0 × 10 6 . Data for the intrinsic viscosity [η] may be fitted for M > 10 5 by [η] = K ′ M a , where K ′, a are in 0.2 M NaCl, 0.0228, 0.816; in 0.5 M NaCl, 0.0318, 0.777; in 0.1 M HCl, 0.0279, 0.763, respectively. Below M = 10 5 , [η] appears to fall below the values calculated, possibly due to non‐Gaussian behavior of short chains. The second virial coefficient A 2 in 0.5 M NaCl approaches the ideal Donnan value, decreasing slightly with increasing molecular weight. The friction parameter P is about 20% smaller than that of uncharged polymers of similar dimensions. The flexible coiling nature of this polymer is confirmed.