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Simplified quantitative electron microscopy of biopolymers
Author(s) -
Lang D.,
Mitani Michiko
Publication year - 1970
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1970.360090310
Subject(s) - chemistry , macromolecule , dna , adsorption , formaldehyde , electron microscope , molecule , microscopy , chemical physics , biophysics , crystallography , chemical engineering , biochemistry , organic chemistry , optics , physics , biology , engineering
Shape and molecular weight of DNA can be obtained with a new method involving spontaneous surface adsorption of DNA or of other macromolecules from a solution which contains formaldehyde and a critical amount of cytochrome c. Subsequent transfer to specimen grids and electron microscopy shows individual molecules suitable for quantitative evaluation. Adsorption of DNA and fd bacteriophages is proportional to (time) 0.75 . Contour lengths of double‐stranded DNA are not influenced by formaldehyde. A modification of this method requires only 2 × 10 −5 μg DNA.