Determination of stability of the DNA double helix in an aqueous medium | Zendy

Privalov P. L. | Zendy; Ptitsyn O. B. | Zendy; Birshtein T. M. | Zendy

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Determination of stability of the DNA double helix in an aqueous medium

Author(s) -

Privalov P. L.,

Ptitsyn O. B.,

Birshtein T. M.

Publication year - 1969

Publication title -

biopolymers

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.556

H-Index - 125

eISSN - 1097-0282

pISSN - 0006-3525

DOI - 10.1002/bip.1969.360080502

Subject(s) - chemistry , ionic strength , dna , aqueous solution , ionic bonding , denaturation (fissile materials) , native state , helix (gastropod) , base pair , base (topology) , crystallography , thermodynamics , organic chemistry , biochemistry , nuclear chemistry , ion , ecology , physics , mathematics , biology , snail , mathematical analysis

The possibility of determining the free energy of stabilization Δ G 0 of native DNA structure with the help of calorimetric data on heats Δ H of transition from the native to denaturated state is considered. Results of microcalorimetric measurements of heats of denaturation of T 2 phage DNA at, different values of pH and ionic strength of solution are given. Values of free energy of stabilization of the DNA native structure Δ G 0 under various conditions have been obtained. It is shown that under conditions close to physiological Δ G 0 approaches 1200 cal/mole per base pair.

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