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Determination of stability of the DNA double helix in an aqueous medium
Author(s) -
Privalov P. L.,
Ptitsyn O. B.,
Birshtein T. M.
Publication year - 1969
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1969.360080502
Subject(s) - chemistry , ionic strength , dna , aqueous solution , ionic bonding , denaturation (fissile materials) , native state , helix (gastropod) , base pair , base (topology) , crystallography , thermodynamics , organic chemistry , biochemistry , nuclear chemistry , ion , ecology , physics , mathematics , biology , snail , mathematical analysis
The possibility of determining the free energy of stabilization Δ G 0 of native DNA structure with the help of calorimetric data on heats Δ H of transition from the native to denaturated state is considered. Results of microcalorimetric measurements of heats of denaturation of T 2 phage DNA at, different values of pH and ionic strength of solution are given. Values of free energy of stabilization of the DNA native structure Δ G 0 under various conditions have been obtained. It is shown that under conditions close to physiological Δ G 0 approaches 1200 cal/mole per base pair.