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Mise en évidence d'une relation entre la longueur el la constante de sédimentation des molécules de DNA en solution
Author(s) -
Nicolaíeff Anatole,
Litzler Robert
Publication year - 1969
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1969.360080205
Subject(s) - chemistry , sedimentation , dna , electron microscope , crystallography , microbiology and biotechnology , analytical chemistry (journal) , physics , chromatography , optics , geomorphology , biochemistry , biology , sediment , geology
A comparative study on sonicated calf thymus DNA was made by using electron microscopy and sedimentation methods. The length distribution, established by electron microscopy by using Kleinschmidt's technique, is related to the sedimentation curve obtained with the same solution. The relation permits a determination of the sedimentation constant corresponding to a given length up to 8000 Å and vice versa.